Overexpression of cyclin D1 is frequently found in various types of human solid tumors, and results from clonal rearrangement and/or amplification involving chromosomal region 11q13. Cyclin D1/PRAD 1 is a likely "driver gene" for this amplicon,
due
to
role in cell cycle control and because it has been specifically implicated as an oncogene in parathyroid adenomas and B cell lymphomas with 11q13 translocation breakpoints. In order to evaluate the pathologic relevance of cyclin D1 overexpression
in
human prostatic adenocarcinoma we detected overexpression of this protein in formalin-fixed, paraffin-embedded tumor materials using polyclonal antibody. For the nuclear protein exposure, micorwave/citrate buffer antigen retrieval system was
processed,
as cell Cyclin D1 antigen is overexpressed in the nucleus and /or cytoplasm of cancer cells. Strong positive reactions are noted in case of stage D1 and 2 cases of stage D2, and the relationship between clinical stage of prostatic adenocarcinoma
and
cyclin D1 overexpression shows that more advanced cases tend to overexpress Cyclin D1(χ(?)=11.749, p=0.068), although the number of cases is not enough. We modified Gleason's grading system, that is grouping into grade I(Gleason's sum score
2~5),
grade
II(Gleason's sum score 6 or 7) and grade III(Gleason's sum score 8~10). According this system in the higher histologic grade, cyclin D1 shows more overexpression (χ(?)=12.928, p=0.012). These results suggest that cyclin D1 overexpression may
play
an
important role in carcinogenesis of advanced prostatic adenocarcinoma and cyclin D1 overexpression may be one of the useful and important prognostic factor in prostatic adenocarcinoma, even though unclear.
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